Forschungsbericht 2009

V-6 Technische Biokatalyse

Forschungsprojekte

• E.2-10.006 Reaktionstechnische Untersuchungen zu Thiamindiphosphat-abhängigen C-C-knüpfenden Enzymen
• E.2-10.007 Entwicklung asymmetrischer biokatalytischer Verfahren unter nicht-konventionellen Reaktionsbedingungen
• E.2-10.009 Oxidation von Hydroxyverbindungen mit Oxidoreduktasen
• E.2-10.010 Weiterentwicklung eines Verfahrens zur Herstellung hochwertiger Leinenfasern durch biotechnische Modifikation
• E.2-10.011 Hydrolasen für Synthese und Hydrolyse von biologisch aktiven reinen Enantiomersubstanzen
• E.2-10.012 Reaction engineering of enantioselective Henry reaction catalysed by hydroxynitrile lyase from Hevea brasiliensis
• E.2-10.013 Entwicklung eines biokatalytischen Prozesses für die Herstellung enantiomerenreiner beta-Aminosäuren durch Michael Addition von Stickstoffnukleophilen
• E.2-10.014 Enantioselektive Katalyse unter Anwendung hoher Drücke
• E.2-10.015 Entwicklung eines Brauverfahrens zur Gewinnung eines nichtalkoholischen Getränkes und Optimierung des Prozesses unter besonderer Berücksichtigung des hierbei auftretenden Stofftransportes
• E.2-10.016 Biokatalyse in hochviskosen Medien
• E.2-10.017 Einsatz neuartiger Partikel in der biotechnologischen Anwendung
• E.2-10.018 Enzymatische, asymmetrische C-C-Bindungsknüpfung mittels Aminosäure- und a-Ketosäure Decarboxylasen zur Synthese relevanter Intermediate für die chemisch - pharmazeutische Industrie
• E.2-10.019 Entwicklung eines biokatalytischen Verfahrens zur Produktion von Cellulose-Estern
• E.2-10.020 Enzymimmobilisierung und Oberflächenwechselwirkung der bakteriellen Glukose-6-phosphatdehydrogenase
• E.2-10.021 Entwicklung eines biokatalytischen Prozesses zur Herstellung von Biowasserstoff

Kooperationen

• Abteilung für allgemeine Mikrobiologie und Biotechnologie, Universität Hamburg
• Bayer Material Science, Leverkusen
• Boehringer Mannheim
• DSM, NL
• Dept. Biocatalysis and Organic Chemistry, Delft University of Technology , NL
• European Molecular Biology Laboratory (EMBL) c/o DESY, Hamburg
• Evocatal, Düsseldorf
• FA. Sartorius
• FA. Surflay-Nanotec
• FAU Erlangen-Nürnberg Institut für Organische Chemie
• Goldschmidt AG
• Institut für Biochemie, Universität Potsdam
• Institut für Bioprozess- und Bioverfahrenstechnik, TUHH
• Institut für Biotechnologie II, Forschungszentrum Jülich
• Institut für Chemische Biotechnologie, Universität Dortmund
• Institut für Feststoffverfahrenstechnik und Partikeltechnologie, TUHH
• Institut für Mikrotechnik (IMM), Mainz
• Institut für Molekulare Enzymtechnologie, Heinrich-Heine Universität Düsseldorf
• Institut für Organische Chemie, Christian-Albrechts-Universität zu Kiel
• Institut für Organische Chemie, Freie Universität Berlin
• Institut für Organische Chemie, TU Graz, A
• Institut für Organische Chemie, Universität Münster
• Institut für Organische und Bioorganische Chemie, Karl-Franzens Universität Graz, A
• Institut für Prozess- und Anlagentechnik, TUHH
• Institut für Technische Chemie, Bereich Wasser- und Geotechnologie, Forschungszentrum Karlsruhe
• Institut für Technische Chemie, Forschungszentrum Karlsruhe
• Institut für Technische Chemie, Universität Hanover
• Institut für Technische Mikrobiologie, TUHH
• Institut für Textilverfahrenstechnik (ITV), Holstein Flachs, Lauffenmühle
• Institut für Thermodynamik, Helmut-Schmidt Universität Hamburg
• Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia
• Lehrstuhl für Pharmazeutische und Medizinische Chemie, Albert-Ludwigs Universität Freiburg
• Max-Planck-Arbeitsgruppe für strukturelle Molekularbiologie und Arbeitsgruppe Proteindynamik, DESY Hamburg
• Sigma-Aldrich Corporation, Buchs, Schweiz
• Zentrum für Bioinformatik, Universität Hamburg

Publikationen

Veröffentlichungen

• 2-10.126V
  

  B.Böckle, B.Galunsky, R.Müller (1995) Chracterisation of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530 Appl. Environ. Microbiol. 61, 3705-3710

• 2-10.130V
  

  O.Guthmann, R.Schwerdtfeger, A.Rieks, G.Antranikian, V.Kasche, G.Brunner (1996) Enzyme catalysed reactions, enantioselectivity and stability under high hydrostatic pessure in: High pressure chemical engineering (Ph. Von Rohr and Ch. Trepp eds.), Elsivier, 127-131

• 2-10.133V
  

  V. Kasche (1996) An integrated approach in the analytical desorption of of affinity chromatography, biosensors, immobilised enzymes and and similar systems Adv. Mol. Cell. Biol. 15B, 605-620

• 2-10.134V
  

  Z. Mincheva, N. Stambolieva, K. Petrova, and B. Galunsky (1996) Penicillin amidase-catalysed preparative synthesis of cephem 7-(2-benzoxazolon-3-yl-acetamido)-desacetoxycephalosporanic acid using a nonspecific polyethyleneglycol-modified acyl donor Biotechnol. Tech. 10, 727-730

• 2-10.135V
  

  I.Ivanov, V.Kasche, D.Petkov (1997) Thermodynamics of enzymic peptide synthesis in biphasic aqueous-organic systems: Coupling of the chemical and phase equilibria Biocatal. Biotransformation 14, 181-194

• 2-10.136V
  

  N.Gottschlich, V.Kasche (1997) Purification of monoclonal antibodies by simulated moving-bed chromatography J.Chromatogr. 765, 201-206

• 2-10.137V
  

  B.Galunsky, Z.Ignatova, V.Kasche (1997) Temperature effects on S1- and S1`-enantioselectivity of a-chymotrypsin. Biochem. Biophys. Acta 1343, 130-138

• 2-10.138V
  

  Z.Ignatova, S.Stoeva, B.Galunsky, C.Hörnle, A.Nurk, E.Piotraschke, W.Voelter, V.Kasche (1998) Proteolytic processing of penicillin amidases from Alcaligenes faecalis cloned in E.coli yields several active forms Biotechnol. Lett. 20, 977-982

• 2-10.139V
  

  A.Chikere, B.Galunsky, V.Kasche (1998) Soybean lypoxygenases: Purification and stability of the free and immobilized enzymes in: Progress in Biotechnology 15, 559-564 (A.Ballesteros, F.J.Plou, J.L.Ibora, P.J.Halling eds.) Elsevier, Amsterdam

• 2-10.142V
  

  A.Overmeier, S.Schrader-Lippelt, V.Kasche, G.Brunner (1999) Lipase-catalysed kinetic resolution of racemates at temperatures from 40°C to 160°C in supercritical carbon dioxide Biotechnol. Lett. 21, 65-69

• 2-10.144V
  

  K.Lüdtke, K.-V.Peinemann, V.Kasche, R.-D.Behling (1999) Nitrate removal of drinking water by means of catalytically active membranes J.Membrane Science 151, 3-12

• 2-10.145V
  

  W.Tischer, V.Kasche (1999) Immobilized enzymes: Crystals or carriers? TIBTECH 17, 326-335

• 2-10.146V
  

  L.Hewitt, V.Kasche, K.Lummer, A.Rieks, K.Wilson (1999) Crystalisation of a precursor penicillin acylase from Escherichia coli Acta Crystallogr. 55, 1052-1054

• 2-10.147V
  

  V. Kasche, K. Lummer, A. Nurk, E. Piotraschke, A. Rieks, S. Stoeva, W. Voelter (1999) Intramolecular autoproteopysis initiates the maturation of penicillin amidase from E. coli Biochim. Biophys. Acta 1433, 76-86

• 2-10.148V
  

  K. Lummer, A. Rieks, B. Galunsky, V. Kasche (1999) pH dependence of penicillin amidase enantioselectivity for charged substrates Biochim. Biophys. Acta 1433, 327-334

• 2-10.149V
  

  Z. Ignatova, S.-O. Enfors, S. Hobbie, S. Taruttis, C. Vogt, V. Kasche (2000) The relative importance of intracellular proteolysis and transport on the yield of the periplasmic enzyme penicillin amidase in Escherichia coli Enzyme Microb. Technol. 26, 165-170

• 2-10.151V
  

  B.Böckle, B.Galunsky, R.Müller (1995) Chracterisation of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530 Appl. Environ. Microbiol. 61, 3705-3710

• 2-10.152V
  

  R. Müller, C. Breidenbach and G. Hofmann (1996) Ist der Einsatz von speziell im Labor gezüchteten Mokroorganismen in der Bodensanierung sinnvoll? in: Neue Techniken der Bodenreinigung. Hamburger Berichte 10. (R. Stegmann Hrsg.) Economia Verlag. p 343-360.

• 2-10.153V
  

  A. Mutzel, U.M. Reinscheid, G. Antranikian and R. Müller (1996) Isolation and characterization of a thermophilic bacillus strain, that degrades phenol as sole carbon source at 70°C. Appl. Microbiol. Biotechnol. 46, 593-596.

• 2-10.154V
  

  B. Seibold, M. Matthes, M.H.M. Eppink, F. Lingens, W.J.H. van Berkel and R. Müller (1996) 4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity. Eur. J. Biochem. 239, 469-478.

• 2-10.155V
  

  U. M. Reinscheid, M. Bauer and R. Müller (1997) Biotransformation of halophenols by a thermophilic Bacillus sp. Biodegradation, 7, 455-461.

• 2-10.156V
  

  B. Böckle and R. Müller (1997) Reduction of disulfide bonds by Streptomyces pactum during growth on chicken feathers. Appl. Environ. Microbiol. 63, 790-792.

• 2-10.157V
  

  F. M. Duffner, U. M. Reinscheid, M. P. Bauer, A. Mutzel and R. Müller (1997) Strain differentiation and taxonomic characterisation of a thermophilic group of phenol-degrading bacilli. System. Appl. Microbiol. 20, 602-611.

• 2-10.158V
  

  R. Müller, G. Antranikian, S. Moloney and R. Sharp (1998) Thermophilic degradation of environmental pollutants. Adv. Biochem. Eng. Biotechnol. 61, 156-169

• 2-10.159V
  

  F. M. Duffner and R. Müller (1998) A novel phenol hydroxylase and catechol 2,3-dioxygenase from the thermophilic Bacillus thermoleovorans strain A2: nucleotide sequence and analysis of the genes. FEMS Micribiol. Lett. 161, 37-45.

• 2-10.160V
  

  U. Rode and R. Müller (1998) Transformation of the Ionic X-ray Contrast Agent Diatrizoate and Related Triiodinated Benzoates by Trametes versicolor Appl. Environ. Microbiol. 64, 3114-3117.

• 2-10.161V
  

  C. Wischnak, F. E. Löffler, J. Li, J. W. Urbance, and R. Müller (1998) Pseudomonas sp. Strain 273, an Aerobic Dichloroalkane-Degrading Bacterium. Appl. Environ. Microbiol. 64, 3507-3511.

• 2-10.162V
  

  N. Stambolieva, Z. Mincheva, B.Galunsky (1998) Kinetic comparison of penicillin amidase catalysed transfer of nonspecific and specific acyl moieties to 7-aminodeacetoxycephalosporanic acid Biocatal. Biotransform. 16, 225-232

• 2-10.163V
  

  R.E. Milo, F.M. Duffner, R. Müller (1999) Catechol 2,3-dioxygenase from the thermophilic phenol-degrading Bacillus thermoleovorans strain A2 has unexpected low thermal stability Extremophiles 3, 185-190

• 2-10.164V
  

  R. Müller (1999) Grundlagen des mikrobiologischen Schadstoffabbaus VDI Berichte 1478, 381-396

• 2-10.166V
  

  P. J. Sträßner, L.-E. Gminder und R. Müller (2000) Optimierung des mikrobiellen Abbaus von Polyvinylalkohol (PVA) in einer Modellkläranlage Wasser/Abwasser 140, 280-285

• 2-10.165V
  

  U. M. Reinscheid, H. Zuilhof, R. Müller and J. Verwoort (1999) Biological, thermal and photochemical transformation of trifluoromethylphenol Biodegradation 9, 487-499

• 2-10.167V
  

  B. Galunsky, K. Lummer, V. Kasche (2000) Comparative study of substrate- and stereospecificity of penicillin G amidases from different sources and hybrid enzymes Chem. Montl. 131, 623-632

• 2-10.168V
  

  Z. Ignatova, S. Taruttis, V. Kasche (2000) Role of the intracellular proteolysis in the production of the periplasmic enzyme penicillin amidase in Escherichia coli Biotechnol. Lett. 22, 1727-1732

• 2-10.169V
  

  V. Kasche (2001) Proteases in peptide synthesis in: Proteases a practical approach (I. Bond and R. Beynon eds.), 2nd edition, Oxford Univ. Press, Oxford, 265-292

• 2-10.170V
  

  A. Chikere, B. Galunsky, V. Schünemann, V. Kasche (2001) Stability of immobilised soybean lipoxygenases: influence of coupling conditions on the ionisation state of the active site Fe Enzyme Microb. Technol. 28, 168-175

• 2-10.171V
  

  V. Kasche, A. Spiess (2001) Enzymatic transformations in suspensions in: Methods in Biotechnology Vol.15: Enzymes in Nonaqueous Solvents (E. Vulfson, P. Halling, B. Holland eds.), Humana Press Inc., 553-563

• 2-10.172V
  

  K. Buchholz, V. Kasche, U.T. Bornscheuer (2005) Biocatalysis and Enzyme Technology. Wiley-VCH

• 2-10.173V
  

  L. Hewitt, V. Kasche, K. Lummer, R.J. Lewis, G.N. Murshudov, G.G. Dodson and K.W. Wilson (2000) Structure of slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active site cleft J. Mol. Biol. 302, 887-898

• 2-10.174V
  

  A. Chikere, B. Galunsky, A. Overmeyer, G. Brunner, V. Kasche (2000) Activity and atability of immobilised soybean lipoxygenase-1 in aqueous and supercritical carbon dioxide media Biotechnol. Lett. 22, 1815-1821

• 2-10.175V
  

  F.M. Duffner, U. Kirchner, M.P. Bauer, R. Müller (2000) Gene 256, 215-221

• 2-10.176V
  

  C. Wischnak, R. Müller (2000) in Biotechnology (Environmental processes) Vol 11b (J.Klein ed.), VCH, pp. 241-272

• 2-10.181V
  

  H. Feitkenhauer, S. Schnicke, R. Müller, H. Märkl (2001) Determination of the kinetic parameters of the phenol-degrading thermophile Bacillus thermoleovorans sp.A2 Appl. Microbiol. Biotechnol.57, 744-750

• 2-10.177V
  

  Z. Ignatova, S.-O. Enfors, M. Hobbie, S. Taruttis, C. Vogt, V. Kasche (2001) The relative importance of intracellular proteolysis and transport on the yield of the periplasmatic enzyme penicillin amidase in Escherichia coli Enzyme Microb. Technol. 26, 165-170

• 2-10.178V
  

  A.J.J.Straathof, V.Kasche (2001) Process conceptions for bioconversions in Applied Biocatalysis (A.J.J. Straathof and P. Adlercreutz eds.) Harwood Academic Publishers, pp. 317-338

• 2-10.179V
  

  A.C. Chikere, B. Galunsky, V. Schünemann, V. Kasche (2001) Stability of immobilised lipoxigenases: influence of coupling conditions on the ionisation state of the active site Fe Enzyme Microbial Technology 28, 168-175

• 2-10.180V
  

  Spiess, V. Kasche (2001) Direct measurement of pH profiles in immobilised enzyme carriers during kinetically controlled synthesis using CLSMBiotechnology Progress 17, 294-303

• 2-10.182V
  

  V. Kasche (2001) Physico-chemical boundaries in the continuous and one-step discontinuous affinity-chromatographic isolation of proteins J. Biochem. Biophys. Meth. 49, 49-62

• 2-10.183V
  

  A. Spiess, V. Kasche (2001) Enzymatic synthesis of amoxicillin. Process integration using multiphase systems in Novel Frontiers in the Production of Compounds for Biomedical Use (A. van Broekhoven et al., eds.) Kluwer Academic Publishers, Amsterdam, 169-192

• 2-10.184V
  

  B. Mahro, R. Müller, V. Kasche (2001) Bioavailability-the key factor of soil bioremediation in Tratment of Contaminated Soil (R.Stegmann et al., eds.) Springer, 181-196

• 2-10.185V
  

  R. Müller, B. Mahro (2001) Bioaugmentation: Advantages and problems using micro organisms with special abilities in soil decontamination in Tratment of Contaminated Soil (R.Stegmann et al., eds.) Springer, 325-342

• 2-10.186V
  

  Z. Ignatova, C. Hörnle, A. Nurk, V. Kasche (2002) Unusual signal peptide directs penicillin amidase from E. coli to the Tat translocation machinery Biochem. Biophys. Res. Comm. 291, 146-149

• 2-10.187V
  

  B. Galunsky, V. Kasche (2002) Determination of the enantioselectivity for kinetically controlled condensations catalysed by amidases and peptidases. Adv.Synth.Catal. 344, 1115-1119

• 2-10.188V
  

  Z. Ignatova, A. Mahsunah, M. Georgieva, V. Kasche (2003) Improvement of post-translational bottlenecks in the production of penicillin amidase in recombinant E.coli strains. Appl.Environ.Microbiol. 69, 1237-1245

• 2-10.189V
  

  V. Kasche, M. de Boer, C. Lazo, M. Gad (2003) Direct observation of intraparticle equilibration and the rate limiting step in adsorption of proteins in chromatographic adsorbents with confocal laser scanning microscopy (CLSM). Journal of Chromatography B 790, 115-129

• 2-10.190V
  

  I. Reimann, A. Götsche, R. Müller, H. Märkl (2002) Reinigung fetthaltiger Abwässer der Frostfischindustrie mit thermophilen Mikroorganismen. Chemie Ingenieur Technik 74, 634

• 2-10.192V
  

  V. Kasche, B. Galunsky, Z. Ignatova (2003) Fragments of pro-peptide activate mature penicillin amidase of Alkaligenes faecalis. Eur. J. Biochem. 270, 4721-4728

• 2-10.193V
  

  Kirchner U, Westphal AH, Muller R, van Berkel WJ. (2003) Phenol hydroxylase from Bacillus thermoglucosidasius A7, a two-protein component monooxygenase with a dual role for FAD. J Biol Chem. 278, 47545-53

• 2-10.194V
  

  Bozhinova, D., Galunsky, B., Yueping, G., Franzreb, M., Köster, R., & Kasche, V. (2004) Evaluation of magnetic polymer micro-beads as carriers of for immobilised biocatalysts for selective and stereoselective transformations. Biotechnol. Lett. 26, 343-350.

• 2-10.195V
  

  Guncheva, M., Ivanov, I., Galunsky, B., Stambolieva, N., Kaneti, J. (2004) Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263. Eur. J. Biochem. 271, 2272-2279.

• 2-10.196V
  

  Z. Ignatova, L. Gierasch (2004) Monitoring protein stability and aggregation in vivo by real-time fluorescence labelling. Proc. Natl. Acad. Sci. 101, 523-528.

• V-06.001V
  

  Siwek, M., Galunsky, B., Niemeyer, B. (2005) Isolation of selenium organic species from Antarctic krill after enzymatic hydrolysis. Anal. Bioanal. Chem. 381, 737-741.

• V-06.002V
  

  L. Greiner, S. Laue, A. Liese, C Wandrey (2005) Continuous homogeneos asymmetric-transfer hydrogenation of ketones: Lessons from kinetics. Chemistry A European Journal 12, 1818-1823, DOI: 10.1002/chem 200500675

• V-06.003V
  

  P. Dominguez de Maria, T. Stillger, M. Pohl, S. Wallert, K. Drauz, H. Gröger, H. Trautwein, A. Liese (2006) Preparative enantioselective synthesis of benzoins and (R)-2-hydroxy-1-phenylpropanone by using benzaldehyde lyase. Journal of Molecular Catalysis B: Enzymatic 38, 43-47

• V-06.004V
  

  A. Liese (2005) Technical application of biological principles in asymmetric catalysis. Advances in Biochemical Engineering/Biotechnology 92, 197-224.

• V-06.005V
  

  P. Christophliemk, M. Liauw, A. Liese (2005) Trendbericht Technische Chemie 2004. Nachrichten aus der Chemie 3, 312-316.

• V-06.006V
  

  R. Mertens, A. Liese, C. Wandrey (2005) Reaktionstechnische Aspekte der enzymatischen Herstellung von NADPH im Enzym-Membran-Reaktor. Chemie Ingenieur Technik 77(5), 609-616.

• V-06.007V
  

  Camilo E. La Rotta Hernandez, Stephan Lütz, Andreas Liese, Elba P. S. Bon (2005) Activity and stability of Caldariomyces fumago chloroperoxidase modified by reductive alkylation, amidation and cross-linking. Enzyme and Microbial Technology 37(6), 582-588.

• V-06.008V
  

  A.W.I. Tan, M. Fischbach, H. Huebner, R. Buchholz, W. Hummel, T. Daussmann, C. Wandrey, A. Liese (2006) Synthesis of enantiopure (5R)-hydroxyhexane-2-one with immobilised whole cells of Lactobacillus kefiri. Applied Microbiology Biotechnology 71, 289-293.

• V-06.010V
  

  S. Rappert, R. Müller (2005) Odor compounds in waste gas emissions from agricultural operations and food industries. Waste Management 25, 887-907.

• V-06.011V
  

  S. Rappert, R. Müller (2005) Microbial degradation of selected odorous substances. Waste Management 25, 940-54.

• V-06. 012V
  

  L. Greiner, A. Liese (2005) Hydrogenation and other reduction reactions. In Multiphase Homogeneous Catalysis (Cornils, Herrmann, Vogt, Horvath, Oliver-Bourbigou, Leitner, Mecking, eds.), Wiley-VCH, 819-834.

• V-06.013V
  

  L. Hilterhaus, A. Liese, M. Howaldt, H. Chmiel (2006) Enzymkinetik in Bioprozesstechnik (Chmiel, ed.), Elsevier, 67-97. ISBN 3-8274-1607-8

• V-06.014V
  

  S. Briechle, A. Liese, M. Howaldt, T. Roethig (2006) Enzymatische Prozesse in Bioprozesstechnik (Chmiel, ed.), Elsevier 361-408, ISBN: 3-8274-1607-8.

• V-06.015V
  

  A. Liese, K. Seelbach und C. Wandrey (2006) Industrial Biotransformations, second edition. VCH-Wiley, Weinheim (Germany), ISBN: 3-527.31001-0.

• V-06.016V
  

  Kasche, V., Ignatova, Z., Märkl, H., Plate, W., Punckt, N., Schmidt, D., Ernst, B (2005) Ca2+ is required for transport and maturation and is yield determining factor in high cell density fermentations of penicillin amidase. Biotechnol. Prog. 21, 432-438.

• V-06.017V
  

  Ignatova, Z., Wischnewski, F., Notbohm, H., and Kasche, V. (2005) Pro-sequence and Ca2+-binding: implications for the folding and activity of penicillin amidase. J. Mol. Biol. 348, 999-1014.

• V-06.018V
  

  Viegas, S., Schmidt, D., Arraiano, C.M., Kasche, V. and Ignatova, Z. (2005) Increased stability of penicillin amidase mRNA in RNase deletion strains is differently sequestered at post-translational level. FEBS Lett. 579, 5069-5073.

• V-06.019V
  

  Ignatova, Z. and Gierasch, L.M. (2005) Aggregation of a slow-folding mutant of a beta-clam protein proceeds through a monomeric nucleus. Biochemistry 44, 7266-7274.

• V-06.020V
  

  Ignatova, Z. (2005) Monitoring protein stability in vivo. (Review) Microb. Cell. Fact. 4, 1-6.

• V-06.021V
  

  Martinez-Perez, I.M., Ignatova, Z., Zhang, G. and Zimmermann, K.-H. (2005) Solving the Hamiltonian path problem via DNA hairpin formation. Int. J. Bioinformatics Res. Appl. 1, 389-398.

• V-06.022V
  

  L. Greiner, S. Laue, J. Wöltinger, A. Liese (2006) Contionuous asymmetric hydrogenation. Topics in Current Chemistry, DOI:10.1007/128_2006_096

• V-06.023V
  

  L. Hilterhaus, A. Liese (2006) Building blocks. Advances in Biochemical Engineering/Biotechnology, available online, DOI:10.1007/10_033

• V-06.024V
  

  K. Goldberg, K. Edegger, W. Kroutil, A. Liese (2006) Overcoming the thermodynamic limitation in assymetric hydrogen transfer reactions catalyzed by whole cells. Biotechnology and Bioengineering, 95:192-198.

• V-06.025V
  

  H. Chakib, S. Roller, M. Beigi, A. Liese, R. Haag (2006) Polyglycerol Cr-Salen as a high-loading dendritic catalyst for stereoselective Diels-Alder reactions. Adv. Synth. Cat. 348, 1760-1771.

• V-06.026V
  

  T. Stillger, M. Pohl, C. Wandrey, A. Liese (2006) Reaction engineering of benzaldehyde lyase catalyzing enantioselective C-C bond formation.  Organic Process Research & Development 10 (6), 1172-1177

• V-06.027V
  

  R. Busch, T. Hirth, A. Liese, S. Nordhoff, J. Puls, O. Pulz, D. Sell, C. Syldatk, R. Ulber (2006) Nutzung nachwachsender Rohstoffe in der industriellen Stoffproduktion. Chemie Ingenieur Technik 78, 219-228.

• V-06.028V
  

  B. Osterath, N. Rao, S. Luetz, A. Liese (2007) Weiße Wäsche, klassischer Käse und Grüne Chemie. Chemie in unserer Zeit 41 (4), 324-333

• V-06.029V
  

  S. Lütz, K. Vuorilehto, A. Liese (2007) Process development for the electroenzymatic synthesis of (R)-methylphenylsulfoxide by use of a 3-dimensional electrode. Biotechnology and Bioengineering 98 (3), 525-534

• V-06.030V
  

  Rappert S, Botsch KC, Nagorny S, Francke W, Müller R. (2006) Degradation of 2,3-diethyl-5-methylpyrazine by a new discovered bacterium Mycobacterium sp. DM-11. Appl. Environ. Microbiol. 72, 1437-1444.

• V-06.031V
  

  Rappert S, Li R, Kokova M, Antholz M, Nagorny S, Francke W & Müller R (2006) Degradation of 2,5-dimethylpyrazine by Rhodococcus erythropolis strain DP-45, isolated from a waste gas treatment plant of a fishmeal processing company. Biodegradation (Epub ahead of print)

• V-06.032V
  

  Rappert S, Ayoub R, Li R, Botsch K, Obi I & Müller R (2004) Biologischer Abbau von geruchsaktiven Einzelstoffen und von realen Vielstoffgemischen. Erfassung und Minimierung von Gerüchen. In: Niemeyer B, Robers A & Thiesen P (Eds) Messung und Minimierung von Gerüchen, Hamburger Berichte 23 (pp. 203-220). Verlag Abfall aktuell, Stuttgart, Germany

• V-06.033V
  

  Siwek, M., Noubar, A.B., Bergmann, J., Niemeyer, B., Galunsky, B. (2006) Enhancement of enzymatic digestion of Antarctic krill and successive extraction of selenium organic compounds by ultrasound treatment. Anal. Bioanal. Chem. 384, 244-249.

• V-06.034V
  

  Siwek, M., Noubar, A.M., Erdmann, R., Niemeyer, B., Galunsky, B. (2008) Application of mixed-mode Oasis MCX adsorbent for chromatographic separation of selenomethionine from Antarctic krill after enzymatic digestion. Chromatographia 67, 305-308.

• V-06.035V
  

  Tishinov, K., Stambolieva, N., Petrova, S., Galunsky, B., B.,  Nedkov, P., (2009)  Purification and characterization of the sunflower seed (Helianthus annuus L.) major aminopeptidase. Acta Physiol Plant  31, 199-205.

• V-06.036V
  

  P. Dominguez de Maria, T. Stillger, M. Pohl, A. Liese, H. Gröger, M. Kiesel, H. Trauthwein (2007)
  Enantionselective C-C Bond Ligation Using Recombinant E. coli-Whole-Cell Biocatalysts. Advanced Synthesis & Catalysis  350 (1) (2008) 165-173

• V-06.037V
  

  K. Goldberg, K. Schroer, S. Lütz, A. Liese (2007) Biocatalytic ketone reduction - a powerful tool for the production of chiral alcohols. Part II: Whole cell reductions. Applied Microbiology and Biotechnology 76 (2), 249-255

• V-06.038V
  

  K. Goldberg, K. Schroer, S. Lütz, A. Liese (2007) Biocatalytic ketone reduction - a powerful tool for the production of chiral alcohols. Part I: Processes with isolated enzymes. Applied Microbiology and Biotechnology 76 (2), 237-248

• V-06.039V
  

  K. Schroer, U. Mackfeld, I. A. W. Tan, C. Wandrey, S. Bringer-Meyer, A. Weckbecker, W. Hummel, T. Daußmann, R. Pfaller, A. Liese, S. Lütz (2007) Continuous Asymmetric Ketone Reduction with Recombinant Escherichia coli. Journal of Biotechnology 132 (4), 438-444

• V-06.040V
  

  Guncheva, M., Ivanov, I., Galunsky, B., Stambolieva, N., Kaneti, J. (2004) Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263. Eur. J. Biochem. 271, 2272-2279.

• V-06.041V
  

  Zhiryakova, D., Ivanov, I., Ilieva, S., Guncheva, M., Galunsky, B., Stambolieva, N. (2009) Are Ntn-Hydrolases Indeed with a Single-Amino-Acid Catalytic Centre? Supporting Amino Acid Residues at the Active Site of Penicillin G Acylase. FEBS J 276, 2589-2598.

• V-06.042V
  

  Tishinov, K., Stambolieva, N., Nedkov, P., Galunsky, B. (2009) Sunflower seed aminopeptidase-catalyzed hydrolysis of Phe-Xaa dipeptides. Exploring the S₁¿ specificity of the enzyme.  J. Mol.Catal.B: Enzym. 59, 106-110.

• V-06.043V
  

  L. Greiner, S. Laue, J. Wöltinger, A. Liese Continuous asymmetric hydrogenation Topics in Current Chemistry, 276 (2007), 111-124. DOI: 10.1007/128_2006_096

• V-06.044V
  

  M. Beigi, R. Haag, A. Liese Continuous application of polyglycerol supported salen in a membrane reactor: Asymmetric epoxidation of 6-cyano-2,2-dimethylchromene  Advanced Synthesis & Catalysis 350 (6) (2008) 919-925.  DOI: 10.1002/adsc.200700591

• V-06.045V
  

  L. Hilterhaus, B. Minow, J. Müller, M. Berheide, H. Quitmann, M.Katzer, O. Thum, G. Antranikian, A. P. Zeng and A. Liese Practical application of different enzymes immobilized on sepabeads Bioprocess and Biosystems Engineering 31 (3) (2008) 183-191.  DOI: 10.1007/s00449-008-0199-3

• V-06.046V
  

   M. Wolberg, M. Villela Filho, S. Bode, P. Geilenkirchen, R. Feldmann, Andreas Liese, W. Hummel, M. Müller
  Chemoenzymatic Synthesis of the Chiral Side-Chain of Statins ¿ Application of an Alcohol Dehydrogenase Catalysed Ketone Reduction on a Large Scale  Bioprocess and Biosystems Engineering 31 (3) (2008) 163-171
  DOI: 10.1007/s00449-008-0205-9

• V-06.047V
  

   K. Goldberg, Anke Krüger, Thomas Meinhardt, Wolfgang Kroutil, Barbara Mautner, A. Liese
  Novel immobilization routes for the covalent binding of an alcohol dehydrogenase from Rhodococcus ruber DSM 44541  Tetrahedron: Asymmetry, 19 (2008) 1171-1173.  DOI: 10.1016/j.tetasy.2008.04.034

• V-06.048V
  

  M. Beigi, S. Roller, R. Haag, A. Liese  Polyglycerol-Supported Co- and Mn-Salen Complexes as Efficient and Recyclable Homogeneous Catalysts for the Hydrolytic Kinetic Resolution of Terminal Epoxides and Asymmetric Olefin Epoxidation  European Journal of Organic Chemistry (12) (2008) 2135-2141. DOI: 10.1002/ ejoc. 200701230

• V-06.049V
  

   L. Hilterhaus, O. Thum, A. Liese  Reactor Concept for Lipase-Catalyzed Solvent-Free Conversion of Highly Viscous Reactants Forming Two-Phase Systems  Organic Process Research Development 12 (4) (2008) 618-625. DOI: 10.1021/op800070q

• V-06.050V
  

   F. R. P. Oliveira, K. Goldberg, A. Liese, B. Hitzmann  Chemometric modelling for process analyzers using just a single calibration sample  Chemometrics and Intelligent Laboratory Systems (online available)  DOI: 10.1016/j.chemolab.2008.07.001, 94 (2) (2008) 118-122

• V-06.051V
  

  N. Kurlemann, A. Liese  Asymmetric synthesis of chiral 2-hydroxy ketones by coupled biocatalytic alkene oxidation and C-C bond formation  Journal of Molecular Catalysis B, Enzymatic 61 (2008) 111-116
  DOI: 10.1016/j.molcatb.2008.08.009

• V-06.052V
  

   F. Hollmann, Y. Gumulya, C. Tölle, A. Liese, O. Thum  Evaluation of the Laccase from Myceliophthora thermophila as Industrial Biocatalyst for Polymerization Reactions  Macromolecules 41 (22) (2008) 8520-8524
  DOI: 10.1021/ma801763t
  
  

• V-06.053V
  

  M. Berheide, S. Peper, S. Kara, W. Sing Long, S. Schenkel, B. Niemeyer, M. Pohl, A. Liese  Influence of the Hydrostatic Pressure and pH on the Asymmetric a-Hydroxyketone Formation Catalyzed by Pseudomonas putida Benzoylformate Decarboxylase and Variants   Biotechnology & Bioengineering 106 (1) (2010) 18-26

• V-06.054V
  

  M. Bruning, M. Berheide, D. Meyer, Ralph Golbik, H. Bartunik, A. Liese, K. Tittmann  Structural and kinetic studies on native intermediates and an intermediate analogue in benzoylformate decarboxylase reveal a least-motion mechanism with an unprecedented short-lived pre-decarboxylation intermediate  Biochemistry 48 (15) (2009) 3258-3268.  DOI: 10.1021/bi801957d

• V-06.055V
  

  N. Richter, M Neumann, A. Liese, R. Wohlgemuth, T. Eggert, W. Hummel Characterisation of a recombinant NADP-dependent glycerol dehydrogenase from Gluconobacter oxydans and its application in the production of L-glyceraldehyde  ChemBioChem 10 (11) (2009) 1888-1896

• V-06.056V
  

  R. Yuryev, A. Liese Synthesis of novel unsymmetrical bisoxazoline ligand with sp² bridge carbon  SYNLETT 16 (2009) 2589-2592

• V-06.057V
  

  R. Yuryev, A. Liese  Why Biocatalysis is Outlaw?  ChemCatChem 2 (1) (2009) 103-107

• V-06.058V
  

  P. Krause, L. Hilterhaus, G. Fieg, A. Liese, U. Bornscheuer  Chemically and enzymatically catalyzed synthesis of C₆-C₁₀ alkyl benzoates  European Journal of Lipid Science and Technology 111 (2009) 194-201
  DOI: 10.1002/ejlt.200800135

• V-06.059V
  

  J. Tao, G.-Q. Lin, A.  Liese Biocatalysis for the Pharmaceutical Industry Wiley-VCH, 2009, 1^st edition, ISBN 0-470-82314-3

• V-06.060V
  

  L. Hilterhaus, A. Liese  Applications of reaction engineering to industrial biotransformations
  In: Biocatalysis for the Pharmaceutical Industry (Tao, Lin, Liese eds.) Wiley (2009), 65-88
  ISBN 0-470-82314-3 

• V-06.061V
  

  Tishinov, K., Stambolieva, N., Petrova, S., Galunsky, B., Nedkov, P.  Purification and characterization of the sunflower seed (Helianthus annuus L.) major aminopeptidase. Acta Physiol Plant 31 (2009) 199-205

• V-06.062V
  

  Tishinov, K., Stambolieva, N., Nedkov, P., Galunsky, B.  Sunflower seed aminopeptidase-catalyzed hydrolysis of Phe-Xaa dipeptides. Exploring the S₁¿ specificity of the enzyme.  J Plant Biochem Biotechnol 18 (2009) 237-239

• V-06.063V
  

  Ruslan Yuryev, Volker Kasche, Zoya Ignatova and Boris Galunsky Improved A. faecalispenicillin amidase mutant retains the thermodynamic and pH stability of the wild type enzyme. The Protein Journal (2010) DOI 10.1007/s10930-010-9238-4

• V-06.064V
  

  Jakob Müller, Markus Neumann, Paul Scholl, Lutz Hilterhaus, Marrit Eckstein, Oliver Thum and Andreas Liese Online Monitoring of Biotransformations in High Viscous Multiphase Systems by Means of FT-IR and Chemometrics. Anal. Chem., DOI: 10.1021/ac100469t (June 2010)

• V-06.065V
  

   A. G. Valladares Juárez, J. Dreyer, P. K. Göpel, N. Koschke, D. Frank, H. Märkl, R. Müller Characterisation of a new thermoalkaliphilic bacterium for the production of high-quality hemp fibres, Geobacillus thermoglucosidasius strain PB94A. Appl Microbiol Biotechnol (2009) DOI 10.1007/s00253-009-1969-9

Dissertationen

• 2-10.006D
  

  Brigitte Böckle: Untersuchungen zum mikrobiellen Abbau von Kreatin. Isolierung und Charakterisierung einer keratinolytisch wirkenden Protease aus Streptomyces pactum, TUHH 1995

• 2-10.007D
  

  Elke Piotraschke: Untersuchungen zur Prozessierung der Penicillin G-Amidase aus Escherichia coli ATCC 11105, TUHH 1995

• 2-10.008D
  

  Jörg Schmieding: Herstellung, Charakterisierung und Anwendung von monoklonalen und polyklonalen Antikörpern für Enzymtechnik und Umweltanalytik, TUHH 1995

• 2-10.009D
  

  Petra Sträßner: Optimierung des mikrobiellen Abbaus von Polyvinylalkohol, TUHH 1995

• 2-10.010D
  

  Annette Eschenbach: Einfluß von Preurotus ostreatus, Kompost, Sphingomonas

  paucimobilis und der Kontaminationsdauer auf den Verbleib und Abbau 14C-markierter polyzyklischer Aromatischer Kohlenwasserstoffe (PAK) in Altlastböden, TUHH 1995

• 2-10.011D
  

  Caroline Breidenbach: Der Abbau von 4-Chlorbenzoat durch Pseudomonas sp. CBS3 im Boden und in Flüssigkultur, TUHH 1995

• 2-10.012D
  

  R.-Ch. Schlothauer: Einfluss von pH-Gradienten in Biokatalysatoren auf Reaktionskinetik und -Ausbeute: Enzymträgervergleich und Modellgestützte Optimierung am Beischpiel der enzymatischen Benzylpenicillin Hydrolyse, TUHH 1996

• 2-10.013D
  

  Ulrike Matena: Der Abbau von Röntgenkontrastmitteln durch den Weißfäulepilz

  Trametes Versicolor, TUHH 1996

• 2-10.014D
  

  Uwe Reinscheid: Untersuchungen zum thermophilen mikrobiellen Abbau von Phenol und Chlorphenolen, TUHH 1997

• 2-10.015D
  

  Gisela Hofmann: Synergistische und antagonistische Effekte beim bakteriellen

  Abbau von Chlorphenolen in Boden und Flüssigkultur, TUHH 1998

• 2-10.016D
  

  Michel Bauer: Der Abbau von Phenolen durch thermophile Mikroorganismen,

  TUHH 1998

• 2-10.017D
  

  Stefan Taruttis: Proteilytische Prozessierung und enzymatische Charaktrisierung natürlicher und rekombinanter Penicillinamidasen in Escherichia coli, 1997, Schaker Verlag, ISBN 3-8265-3239-2, Aachen, 1997

• 2-10.018D
  

  Andre Rieks: Stereospezifität und chirale Erkennung von Enzymen: Optimierung der Enantioselektivität von Penicillinamidase durch Variation der Reaktionsparameter und der Primärstruktur, TUHH, 1997

• 2-10.019D
  

  Kerstin Lüdtke: Herstellung und Charakterisierung katalytischer Membranen für die Nitratreduktion, TUHH, 1997

• 2-10.020D
  

  Norbert Gottschlich: Einsatz der simulierten Gegenstromchromatographie zur Reinigung hochmolekularer Bioprodukte, Schaker Verlag, ISBN 3-8265-3367-4, Aachen, 1998

• 2-10.021D
  

  Leyla-Manuela Schmidt: Aktivität abbauaktiver Mikroorganismen und Enzyme im

  Altlastboden, TUHH, 1998

• 2-10.022D
  

  Rosvita-Edit Milo: Catehol-2,3-Dioxygenase aus dem thermophilen Phenol abbbauenden Bacillus thermoleovorans A2, TUHH 1999

• 2-10.023D
  

  Babette Scheidat: Bioverfahrenstechnische Aspekte zum Einsatz von technischen Enzymen am Beispiel der kommunalen Abwasserreinigung, TUHH 1999

• 2-10.024D
  

  Antje Spieß: The kinetically controlled synthesis of amoxicillin: A penicillin amidase catalysed reaction with suspended substrate and product, TUHH 2000

• 2-10.025D
  

  Karsten Lummer: Inter- und intramolekulare enzymatisch katalysierte Reaktionen am Beispiel der Penicillinamidase, TUHH 2000

• 2-10.026D
  

  Anthony Chikere: Soybean lypoxygenases: Purification and stabilisation for biotransformations in aqueous and supercritical carbon dioxide media, TUHH 2000

• 2-10.032D
  

  Ahmed Abdel-Megeed: Psychrophilic degradation of long chain alkanes, TUHH 2004

• 2-10.031D
  

  Jost Schilbach: Entwicklung und optimiering eines biotechnoligischen Verfahrens zur Herstellung von Proteinschaummitteln, TUHH 2004

• 2-10.030D
  

  Anja Göttsche: Die toxische Wirkung von Ölsäure auf Geobacillus thermoleovorans IHI-91 und Strategien zur Problemlösung, TUHH 2005

• 2-10.027D
  

  Ulrike Kirchner: A novel two-component phenolhydrolyse from Bacillus thermoglucosidasikus, TUHH 2000

• 2-10.028D
  

  Zoya Ignatova: Penicillin amidase a model system to study the post-translational processing influencing the yield of periplasmic enzymes, TUHH 2001

• 2-10.029D
  

  Katja Otto: Characterisation of thermostable alkane oxidising system, TUHH 2001

• 2-10.033D
  

  Daniela Bozhinova: Synthesis, modification and characterisation of magnetic micro-matrices for covalent immobilisastion of biomolecules. Model investigation with penicillin amidase from E. coli, 2004

• V-06.001D
  

  Mariana Siwek: Isolation of Organic Selenium Compounds from Antarctic Krill after Enzymatic Hydrolysis and Bifunctional Chromatography, 2007

• V-06.002D
  

   Maryam Beigi: Sustainability by Advanced Chemoenzymatic Technologies, 2008

• V-06.003D
  

   Lutz Hilterhaus: Development of alternative reactor concepts for the production of novel ester oils, TUHH 2008

• V-06.004D
  

  Nils Kurlemann: Reaction engineering of enzymatic C-C-bond formations towards 2-hydroxy ketones, TUHH 2008

• V-06.005D
  

  Katja Goldberg: Entwicklung eines biokatalytischen Verfahrens zur asymmetrischen Produktion von Alkoholen, TUHH 2010

• V-06.006D
  

   Ana Gabriela Valladares Juarez:                   ,TUHH 2010

• V-06. 007D
  

  Marc Brunning: Structural-functional studied of chorismate synthase and glutamyl tRNAsynthetase from M. tuberculosis, TUHH 2008

Habilitationen

• 2-10.002H
  

  Z. Ignatova (2004) Post-trnascriptional and post-translational processes for regulation of protein function, activity and yield. TUHH.

Patente

• V-06.001P
  

  Martinez-Perez, I.M., Ignatova, Z., Zimmermann, K.-H. (2005) Computational Genes, EU Patent, pending.

• 2-10.001P
  

  Kasche, V., Ignatova, Z., Galunsky, B. (2004) Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung. DE 102 51 747 A1, 19.05.2004.

• V-06.002P
  

  O. Thum, L. Hilterhaus, A. Liese Verfahren zur heterogenkatalysierten Herstellung von Carbonsäurederivaten
  EP2080806, DE102008004725, CN101532035 (2008)

• V-06.003P
  

  O. Thum, L. Hilterhaus, A. Liese Verfahren zur enzymatischen Herstellung von Carbonsäureestern
  EP2080807, JP2009165474, DE102008004726, CN101487030, US2009181439 (2008)

• V-06.004P
  

   T. Stillger, M. Villela, A. Liese, C. Wandrey Verfahren und Vorrichtung zur enzymatischen Co-Faktoren-Regenerierung PT 1.1861 DE 100 60 602 (2005)