Forschungsbericht 2009



V-1 Bioprozess- und Biosystemtechnik

LogoDenickestraße 15
21073 Hamburg

Tel.: 040 / 42878 - 4183
Fax: 040 / 42878 - 2909

e-mail: aze@tuhh.de
www: http://www.tuhh.de/ibb

Professoren

Prof. Dr. rer. nat. An-Ping Zeng (Leitung) ( TUHH )
em. Prof.-Dr.-Ing. Herbert Märkl (TUHH)
   

Sekretariat

Cornelia Hoffmann (TUHH)
   

wissenschaftliche Mitarbeiter/innen

Priv.-Doz. Dr.-Ing. habil. Ralf Poertner (TUHH (Oberingenieur))
Dr. rer.nat. Christiane Goepfert (E-Learning Consortium, AIF)
Dipl.-Biotech David Dietz (TUHH)
M. Sc. Hendrich Quitmann ( TUHH)
Dipl.-Biotech Matthias Wurm (TUHH)
   

sonstige Mitarbeiter/innen

Dipl.-Ing. (FH) Ralf Grajetzki (TUHH)
Jan Sens (TUHH)
Dipl.-Ing. (FH) Thomas Linz (Industrie)
Kerstin Michael (DFG)
   

Gäste

Dr. Marcin Bizukojc ()

 

Forschungsprojekte

 

Kooperationen

  • Agraferm technologies AG
  • Agricultural University of Athens, Greece
  • Bioengineering AG, Wald, Schweiz
  • Bioglobe GmbH, Hamburg
  • Biokraftwerke Fürstenwalde GmbH
  • Dr. H Ma, Prof. I. Goryanin, University Edinburgh, UK
  • Dr. Hansjörg Hauser, Helmholtz-Zentrum für Infektionsforschung, Braunschweig
  • Dr. J. Goncalves, Cambridge University, UK
  • Dr. Jingxiu Bi, Aldelaide University, Australien
  • Dr. Manfred Wirth, Helmholtz-Zentrum für Infektionsforschung, Braunschweig
  • Landwirtschaftliche Fachschule Tulln, Austria
  • National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing
  • Prof. Dr. Irene Wagner-Döbler, Helmholtz-Zentrum für Infektionsforschung, Braunschweig
  • Prof. Dr. Karsten Niehaus, Universität Bielefeld
  • Prof. Dr. S.T. Yang, Ohio State University, USA
  • Prof. Dr. Gheorghe MARIA, Polytechnic University Bucharest
  • Prof. Dr. T. Scheper, Universität Hannover
  • Prof. Dr. Zhi-Long Xiu, Dalian University of Technology, China
  • Prof. Dr.-Ing. Peter Czermak, FH Gießen-Friedberg
  • Prof. Dr.-Ing. Thomas Becker, Uni Stuttgart-Hohenheim
  • Prof. Dr.-Ing. V. Hass, HS Bremen
  • Prof. Jingqi Yuan, Jiaotong University, Shanghai, China
  • Prof. Ying-jin Yuan, Tianjin University, China
  • Universitätsklinikum Eppendorf, Hamburg
  • ZellWerk GmbH, Eichstädt
  • medorex eK, Bovenden
 

Publikationen

Veröffentlichungen

  • 2-09.021V

    Bohmann. A. ; R. Pörtner; J. Schmieding; V. Kasche; H. Märkl: Integrated membrane dialysis bioreactor with radial-flow fixed bed - a new approach for continuous cultivation of animal cells,"Cytotechnology, 9,"51-57, 1992".

  • 2-09.022V

    Bohmann, A. ; R. Pörtner; H. Märkl,"Evaluation of optimal process parameters for the membrane dialysis bioreactor with integrated radial fixed bed; In,"Kaminogawa, S. ; Ametani, A. ; Hachimura, S. ; Animal Cell Technology,"Basic & Applied Aspects, Vol. 5, Kluwer Academic Publishers, 1993. ".

  • 2-09.023V

    Lüdemann, I.; R. Pörtner and H. Märkl,"Effect of NH3 on the cell growth of a hybridoma cell line; Cytotechnology 14,"11-20 (1994) ".

  • 2-09.024V

    Pörtner, R.; A. Bohmann; I. Lüdemann and H. Märkl,"Estimation of specific glucose uptake rates in cultures of hybridoma cells,"Journal of Biotechnology 34,"237-246 (1994)".

  • 2-09.025V

    Ong, C.P.; R. Pörtner; H. Märkl; Y. Yamazaki; K. Yasuda and M. Matsumura,"High density cultivation of hybridoma in charged porous carriers; Journal of Biotechnology 34,"259-268 (1994)".

  • 2-09.029V

    A. Bohmann; R. Pörtner and H. Märkl,"Performance of a membrane dialysis bioreactor with radial-flow fixed bed for the cultivation of a hybridoma cell line; Appl. Microbiol. Biotechnol. 43,"772-780 (1995) ".

  • 2-09.047V

    Pörtner, R.; A. Schilling; I, Lüdemann and H. Märkl: High density fed-batch cultures for hybridoma cells performed with the aid of a kinetic model. Bioprocess Engineering 15 (1996) 117-124

  • 2-09.048V

    Lüdemann, I., R. Pörtner, C. Schaefer, K. Schick, K. Srámková, K. Reher, M. Neumaier, F. Franék and H. Märkl: Improvement of the culture stability of non-anchorage-dependent animal cells grown in serum-free media through immobilization. Cytotechnology 19 (1996) 111-124

  • 2-09.049V

    Reinhold, G., S. Merrath, F. Lennemann and H. Märkl: Modelling the hydrodynamics and the fluid mixing behaviour of a Biogas Tower Reactor. Chem. Eng. Sci. 51,17 (1996) 4065-4073

  • 2-09.050V

    Pörtner, R., I. Lüdemann und H. Märkl: Dialysekulturen mit immobilisierten tierischen Zellen. Biospektrum 6 (1996) 47-49

  • 2-09.051V

    Pörtner, R.; I. Lüdemann and H. Märkl: Dialysis cultures with immobilized hybridoma cells for effective production of monoclonal antibodies. Cytotechnology 23: 39-45 (1997)

  • 2-09.055V

    Pörtner, R.; I. Lüdemann; K. Reher; M. Neumaier; H. Märkl:(1998) Fixed-bed dialysis culture of a transfectoma cell line producing chimeric Fab-fragments with "nutrient-split"-feeding strategy. Biotechnol. Tech. 12,7: 501-505 (1998)

  • 2-09.058V

    Pörtner, R. and H. Märkl: Dialysis cultures. Appl. Microbiol. Biotechnol. 50: 403-414 (1998)

  • 2-09.056V

    Schwabe, J.O.; R. Pörtner and H. Märkl: Improving an on-line feeding strategy for fed-batch cultures of hybridoma cells by dialysis and "nutrient-split"-feeding. Bioproc. Eng. 20, 6: 475 - 484 (1999)

  • 2-09.057V

    Pörtner, R. and Th. Schäfer: Modelling hybridoma cell growth and metabolism - A comparision of selected models and data; Journal of Biotechnology 49: 119-135 (1996)

  • 2-09.059V

    Fassnacht, D.; S. Rössing; N. Ghaussy and R. Pörtner: Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures. Biotechnology Letters 19: 35-38 (1997)

  • 2-09.060V

    Pörtner, R.; S. Rössing; M. Koop and I. Lüdemann: Kinetic studies on hybridoma cells immobilized in fixed bed reactos. Biotechnology and Bioengineering, 55, 3: 535-541 (1997)

  • 2-09.061V

    Pörtner, R.; S. Rössing und D. Fassnacht: Festbettreaktoren mit immobilisierten tierischen und menschlichen Zellen. Bioscope 9: 25-33 (1997)

  • 2-09.062V

    Pörtner, R. and M. Koop: A model for oxygen supply in fixed bed reactors with immobilized hybridoma cells. Bioprocess Engineering 17: 269-275 (1997)

  • 2-09.063V

    Fassnacht, D.; S. Rössing; J. Stange and R. Pörtner: Long-term cultivation of immortalised mouse hepatocytes in a high cell density fixed bed reactor. Biotechnology Techniques 12: 25-30 (1998)

  • 2-09.064V

    Fassnacht, D.; S. Rössing; F. Franék; M. Al-Rubeai and R. Pörtner: Effect of BCL-2 Expression on hybridoma cell growth in serum-supplemented, protein-free and diluted media. Cytotechnology 26 (3): 219-225 (1998)

  • 2-09.065V

    Fassnacht, D.; S. Rössing; R. Singh; M. Al-Rubeai and R. Pörtner: Influence of BCL-2 Expression on antibody productivity in high cell density hybridoma culture systems. Cytotechnology 30: 95-105 (1999)

  • 2-09.066V

    Fassnacht, D. and R. Pörtner: Experimental and theoretical considerations on oxygen supply for animal cell growth in fixed bed reactors. Journal of Biotechnology 72 (3): 169 - 184 (1999)

  • 2-09.067V

    Manusadzianas, L.; R. Vitkas; R. Pörtner and H. Märkl: Phytotoxicities of selected chemicals and industrial effluents to Nitellopsis obtusa cells, assessed by using a rapid electrophysiological Charophyte test. ATLA, 27: 379-386 (1999)

  • 2-09.068V

    Nehring, D., P. Adamietz, N. M. Meenen and R. Pörtner: Perfusion cultures and modelling of oxygen uptake with three-dimensional chondrocyte pellets. Biotechnology Techniques 13: 701-706 (1999)

  • 2-09.069V

    Pörtner, R.; D. Fassnacht and H. Märkl: Immobilization of mammalian cells in fixed bed reactors. BIOforum International 3: 140-141 (1999)

  • 2-09.070V

    Fussenegger, M.; D. Fassnacht; R. Schwartz; J. A. Zanghi; M. Graf, J. E. Bailey and R. Pörtner: Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and decreases DNA release in extended fixed-bed cultivation. Cytotechnology 32: 45-61 (2000)

  • 2-09.092V

    Fassnacht, D.; I. Reimann, R. Pörtner und H. Märkl: Scale-up von Festbettreak-toren zur Kultivierung tierischer Zellen. Chemie Ingenieur Technik 73: 1075 - 1079 (2001)

  • 2-09.093V

    Frahm, B.; H.-C. Blank; P. Cornand; W. Oelßner; U. Guth; P. Lane; A. Munack; K. Johannsen and R. Pörtner: Determination of dissolved CO2 concentration and CO2 production rate of mammalian cell suspension culture based on off-gas measurement. Journal of Biotechnology 99/2: 133-148 (2002)

  • 2-09.094V

    Frahm, B.; P. Lane; H. Atzert; A. Munack; M. Hoffmann; V. Hass and R. Pörtner: Adaptive, model-based control by the Open-Loop-Feedback-Optimal (OLFO) controller for the effective fed-batch cultivation of hybridoma cells. Biotechnology Progress 18: 1095-1103 (2002)

  • 2-09.095V

    Nagel-Heyer, St. and R. Pörtner: Bioreactor cultivation for three-dimensional cartilage implants. G.I.T. Laboratory Journal 2: 66-67 (2002)

  • 2-09.096V

    Frahm, B. und R. Pörtner: Reaktor-Abgasmessungen bei Zellkulturen, BIOforum 4, 2002

  • 2-09.099V

    Frahm, B., Hass, V. C., Lane, P., Munack, A., Märkl, H. and R. Pörtner: Fed-batch Kultivierung tierischer Zellen - eine Herausforderung zur adaptiven, modellbasierten Steuerung, Chemie Ingenieur Technik, vol. 4, pp. 457 460 (2003)

  • 2-09.100V

    Frahm, B., Lane, P., Märkl, H. and R. Pörtner: Improvement of a mammalian cell culture process by adaptive, model-based dialysis fed-batch cultivation and suppression of apoptosis, Bioprocess and Biosystems Engineering, vol. 26, pp. 1-10 (2003)

  • 2-09.098V

    Nehring, D.; P. Czermak; S. Gokorsch und R. Pörtner: Produktion retroviraler Partikel im Festbettreaktor. BioWorld 4: 14-15 (2003)

  • 2-09.103V

    Nehring, D.; R. Gonzales; P. Czermak and R. Pörtner: Mathematical model of a mem-brane filtration process using ceramic membranes to increase retroviral pseudotype vector titer. Journal of Membrane Science 237: 25-38 (2004)

  • 2-09.104V

    Pörtner, R.; J.-O. Schwabe and B. Frahm: Evaluation of selected control strategies for fed-batch cultures of a hybridoma cell line, Biotechnology and Applied Biochemistry 40: 47-55 (2004)

  • 2-09.105V

    Nagel-Heyer, S.; R. Janßen; C. Goepfert; P. Adamietz; N.M. Meenen and R. Pörtner: Carti-lage cultivation on a calcium phosphate ceramic in a bioreactor system. Key Engineering Materials 264-268: 2119-2122 (2004)

  • 2-09.106V

    Janssen, R.; S. Nagel-Heyer; Ch. Goepfert; R. Pörtner; D. Toykan; O. Krummhauer; M.M. Morlock; P. Adamietz; N.M. Meenen; W.M. Kriven; D.-K. Kim; A. Tampieri and G. Celotti: Cal-cium phosphate ceramics as substrate for cartilage cultivation. Ceramic Engineering and Sci-ence Proceedings 25, 4: 523-528 (2004)

  • 2-09.107V

    Pörtner, R.; M. Seemuk; R.-Ch. Schlothauer; D. Elsser: Anaerobe Kultivierung von Lactococcus lactis im Festbettreaktor. Chemie-Ingenieur-Technik, 76, 10: 1599-1602 (2004)

  • 2-09.108V

    Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters, 27(3): 187-192 (2005)

  • 2-09.110V

    Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters 27(3): 187-192 (2005)

  • 2-09.111V

    Frahm, B.; P. Lane, A. Munack und R. Pörtner: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode. Chemie-Ingenieur-Technik, 77 (4), 429 – 435 (2005)

  • 2-09.112V

    Nagel-Heyer, S.; Ch. Goepfert; P. Adamietz; N.M. Meenen; J.-P. Petersen and R. Pörtner: Flow-chamber bioreactor culture for generation of three-dimensional cartilage-carrier-constructs. Bioprocess and Biosystems Engineering, 27: 273-280 (2005)

  • 2-09.113V

    Meenen, N.M., P. Ueblacker, R. Pörtner, C. Göpfert, S. Nagel-Heyer, J. P. Petersen und P. Adamietz: ; „ Knorpel aus dem Labor-eine Sackgasse?“,. Arthroskopie 18: 245-252 (2005)

  • 2-09.114V

    Pörtner, R.; St. Nagel-Heyer; Ch. Goepfert; P. Adamietz and N. M. Meenen: Bioreactor design for tissue engineering. Journal of Bioengineering and Bioscience, 100(3): 235-245 (2005)

  • V-1.001V

    Hass, V.C.; Kuhnen, F.; Schoop, K.-M.; Poertner, R.: Integration of computer based learning and training in bioprocess technical training. Chemie-Ingenieur-Technik, 77(8), 1250-1251 (2005)

  • V-1.002V

    Pörtner, R.; Hass, V.C.: Interactive virtual learning environment for biotechnology (eLearnBioTec). Chemie-Ingenieur-Technik, 77(8), 1256 (2005)

  • V-1.003V

    Nagel-Heyer, St.; Ch. Goepfert; P. Adamietz; N. M. Meenen and R. Pörtner: Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension. Journal of Biotechnology, 121 (4), 486-497 (2006)

  • V-1.004V

    Nehring, D.; R. Gonzales; R. Pörtner and P. Czermak: Experimental and modelling study of different process modes for retroviral production in a fixed bed reactor. Journal of Biotechnology, 122 (2), 239-253 (2006)

  • V-1.005V

    Heyland, J.; K. Wiegandt; Ch. Goepfert; St. Nagel-Heyer; E. Ilinich; U. Schumacher and R. Pörtner: Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure. Biotechnology Letters, 28: 1641-1648 (2006)

  • V-1.006V

    Becker, Th; B. Hitzmann; R. Pörtner; K.F. Reardon; F. Stahl and R. Ulber: Future Aspects of Biopro-cess Monitoring. in: D. Sell, R. Ulber (Eds.); White Biotechnology; Advances in Biochemical Engineer-ing/Biotechnology; Springer Verlag 105 (2007) 249-293

  • V-1.007V

    Petersen, J.-P.; P. Uebelacker; Ch. Goepfert; P. Adamietz; A. Stork; J.M. Rueger; R. Pörtner; M. Amling and N.M. Meenen: Long term results after implantation of tissue engineered cartilage for the treatment of osteochondral lesions in a minipig model. Journal of Materials Science: Materials in Medicin, DOI: 10.1007/s10856-007-3291-3

  • V-1.008V

    R. Pörtner, O.B. Platas, D. Fassnacht, D. Nehring, P. Czermak, H. Märkl: Fixed bed reactors for the cultivation of mammalian cells: design, performance and scale-up. Open Biotechnology Journal, 1: 41-46 (2007)

  • V-1.009V

    Weber, Ch.; S. Pohl; R. Pörtner; Ch. Wallrapp; M. Kassem; P. Geigle and P. Czermak: Expansion and Harvesting of hMSC-TERT. The Open Biomedical Engineering Journal, 1: 38-46 (2007)

  • V-1.010V

    Weber, Ch.; S. Pohl; R. Pörtner; Ch. Wallrapp; M. Kassem; P. Geigle and P. Czermak: Cultivation and Differentiation of Encapsulated hMSC-TERT in a Disposable Small-Scale Syringe-Like Fixed Bed Reactor. The Open Biomedical Engineering Journal, 1: 64-70 (2007)

  • V-1.100V

    C. Ulmer and A.-P. Zeng (2007). Microbial production of 3-hydroxypropionaldehyde from glycerol bioconversion. Chem. Biochem. Eng.  Q. 21; 321-326

  • V-1.101V

    P. Zheng,  J Sun, R. Geffers, A.-P. Zeng (2007) Functional characterization of the gene PA2384 in large scale gene regulation in response to iron starvation in Pseudomonas aeruginosa. J. Biotechnol. 132: 342-352.

  • V-1.102V

    Y.-H. Liu, J.-X. Bi, A.-P. Zeng, J.-Q. Yuan (2007). A population balance model describing the cell cycle dynamics of myeloma cell cultivation. Biotechnol. Prog. 23:1198-1209.

  • V-1.103V

    Pel,H.J., de Winde,J.H., Archer,D.B., Dyer,P.S., Hofmann,G., Schaap,P.J., Turner,G., de Vries,R.P., Albang,R., Albermann,K., Andersen,M.R., Bendtsen,J.D., Benen,J.A., van den,B.M., Breestraat,S., Caddick,M.X., Contreras,R., Cornell,M., Coutinho,P.M., Danchin,E.G., Debets,A.J., Dekker,P., van Dijck,P.W., van Dijk,A., Dijkhuizen,L., Driessen,A.J., d'Enfert,C., Geysens,S., Goosen,C., Groot,G.S., de Groot,P.W., Guillemette,T., Henrissat,B., Herweijer,M., van den Hombergh,J.P., van den Hondel,C.A., van der Heijden,R.T., van der Kaaij,R.M., Klis,F.M., Kools,H.J., Kubicek,C.P., van Kuyk,P.A., Lauber,J., Lu,X., van der Maarel,M.J., Meulenberg,R., Menke,H., Mortimer,M.A., Nielsen,J., Oliver,S.G., Olsthoorn,M., Pal,K., van Peij,N.N., Ram,A.F., Rinas,U., Roubos,J.A., Sagt,C.M., Schmoll,M., Sun,J., Ussery,D., Varga,J., Vervecken,W., van de Vondervoort,P.J., Wedler,H., Wosten,H.A., Zeng,A.P., van Ooyen,A.J., Visser,J., Stam,H. (2007) Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nat.Biotechnol. 25: 221-231.

  • V-1.104V

    F. He, J. Buer, A.-P. Zeng, R. Balling (2007) Dynamic cumulative activity of transcription factors as a mechanism of quantitative gene regulation in multi-regulator transcriptional regulatory motifs. Genome Biology. 8:R181

  • V-1.105V

    J. Sun, X. Liu, U. Rinas and A.-P. Zeng (2007) Metabolic pecularities of Aspergillus niger disclosed by comparative metabolic genomics. Genome Biology. 8:R182

  • V-1.106V

    Q. Zhang, H. Teng, Y. Sun, Z. Xiu and  A.-P. Zeng (2007) Metabolic flux and robustness analysis of glycerol metabolism in Klebsiella pneumoniae. Bioprocess Biosyst Eng. In print.

  • V-1.107V

    M. da Silva, H. Ma, J.Sun, F. He, A.-P. Zeng (2007) Metabolic network. In  B. H. Junker, F.  Schreiber (eds.) Biological Network Analysis, Wiley: 233-253

  • V-1.108V

    B. Ma, F. He, J. Jablonska, S. Winkelbach, W. Lindenmaier, A.-P. Zeng, and K. Dittmar. A system approach for six-colour segmentation analysis of multi-colour images in the infection studies of Listeria monocytogenes. Microscopy Research & Technique. 2007, 70 (2):171-178.

  • V-1.109V

    Ma H., Rosa da Silva M., Sun J., Kumar B., Zeng A.-P. (2007) Reconstruction and Structural Analysis of Metabolic and Regulatory Networks. In: "Introduction to Systems Biology". Eds. Sangdun Choi, Humana Press.

  • V-1.110V

    Zhou F, Bi JX, Zeng AP, Yuan JQ. Macrokinetic model for myeloma cell culture based on stoichiometric balance. Biotechnol Appl Biochem. (2007) 46: 85-95.

  • V-1.111V

    Y. Li, J. Bi, W. Zhou, Y. Huang, L. Sun, A.-P. Zeng,  G. Ma, Z. Su. (2007) Characterization of the large size aggregation of Hepatitis B virus surface antigen (HBsAg) formed in ultrafiltration process. Process Biochemistry.  42: 315-319.

  • V-1.112V

    Q. Zhang, Z.-L. Xiu and A.-P. Zeng (2006) Optimization of microbial production of 1,3-propanediol by Klebsiella pneumoniae under anaerobic and microaerobic conditions by metabolic flux analysis. J. Chem. Ind. Eng. 57, 1403-1409.

  • V-1.113V

    W. Wang, J. Sun, R. Homann, A.-P. Zeng and W-d. Deckwer  (2006) Proteomic characterization of transient expression and secretion of a stress-related metalloprotease in high cell density culture of Bacillus megaterium. J. Biotechnology. 126: 313-324.

  • V-1.114V

    Zheng P, Sun J, van den Heuvel J, Zeng AP. Discovery and investigation of a new, second triose phosphate isomerase in Klebsiella pneumoniae. J Biotechnol. 2006. 125(4):462-473.

  • V-1.115V

    Zeng AP. Editorial. Process Biochemistry (Special Issue: From Biochemical Engineering to Systems Biology - In honour of Dr. Wolf-Dieter Deckwer). 2006. 41(10):2101-2102

  • V-1.116V

    Zheng P, Wereath K, Sun J, van den Heuvel J and Zeng AP. Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae. Process Biochemistry. 2006. 41(10):2160-2169

  • V-1.117V

    Zhou F, Bi J, Zeng AP and Yuan J. A macrokinetic and regulator model for myeloma cell culture based on metabolic balance of pathways. Process Biochemistry. 2006. 41(10):2207-2217

  • V-1.118V

    J. Sun, W. Wang, C. Hundertmark, A.-P. Zeng, D. Jahn and W.-D. Deckwer (2006). A protein database constructed from low-coverage genomic sequence of Bacillus megaterium and its use for accelerated proteomic analysis. J. Biotechnol. 124(3):486-95.

  • V-1.119V

    W.-D. Deckwer, D. Jahn, D. Hempel, A.-P. Zeng (2006) System Biology Approach to Bioprocess Development. Life Science Eng. 6: 455-469.

  • V-1.120V

    W.-D. Deckwer, D. Jahn, A.-P. Zeng, D. Hempel (2006) Systembiologische Ansätze zur Prozessentwicklung. Chem. Ing. Tech. 78, 193-207.

  • V-1.121V

    F. He and A.-P. Zeng (2006) In search of functional association of genes from time-series microarry expression data by a new method based on change trend and expression levels of genes. BMC Bioinformatics 7:69.

  • V-1.122V

    Jan Buer, Astrid M. Westendorf, An-Ping Zeng, Wiebke Hansen & Michael Probst-Kepper (2005) Mechanisms of central and peripheral T cell tolerance: An update. Transfus Med Hemother. 32: (DOI:10.1159/000089128)

  • V-1.123V

    Zhang F., Bi J.X., Zeng A.-P., Yuan J.Q. (2005) A simple kinetic model for myeloma cell growth with lysin as a limiting substrate. J. of Shanghai Jiaotong University. 139, 182-186.

  • V-1.124V

    Zhang F., Bi J.X., Zeng A.-P., Yuan J.Q. (2005) A simple kinetic model for myeloma cell growth with lysin as a limiting substrate. J. of Shanghai Jiaotong University. 139, 182-186.

  • V1-125V

    A.-P. Zeng and J. Bi (2005) Cell Culture Kinetics and Modeling. In: S.S. Ozturk and W.-S. Hu (eds.) Cell Culture Technology for Pharmaceutical and Cellular Therapies. Taylor & Francis Group, Atlanta. pp: 299-347.

  • V1-126V

    B. Kumar, H. Ma and A.-P. Zeng (2005) An integrated cellular network of Escherichia coli and its structural analysis. Proceedings of Foundation of Systems Biology in Engineering. University of California, Santa Babara, USA, August 7-10. pp 107-110.

  • V1-127V

    H. Ma, A.-P. Zeng (2005) Reconstruction of metabolic network from genome information and its structural and functional analysis. In: R. Eils and A. Kriete (Eds.) Computational Systems Biology, Elsevier Inc., USA. Pp: 169-189.

  • V1-128V

    Sun J, Gunzer F, Buer J, Gößling F, Scharfe M, Blöcker H and Zeng AP (2005). Genomic peculiarity of coding sequences and metabolic potential of the probiotic Escherichia coli strain Nissle 1917 inferred from raw genome sequence. J. Biotechnol. 117 (2): 147-161.

  • V1-129V

    A.-P. Zeng, J. Sun, W. Wang, H. Ma and W.-D. Deckwer (2006) Application of genomic and proteomic data for bioprocess analysis and optimisation. In S.T. Yang (ed.) Bioprocessing for value-added products from renewable resources. Elservier Inc, USA. Pp: 25-48. 

  • V1-130V

    E.-J. Kim, W.-D. Deckwer, W. Wang and A.-P. Zeng (2005) Expression of the quorum sensing regulator protein LasR of is strongly affected by iron deficiency and oxygen concentration in Pseudomonas aeruginosa irrespective of cell density. Microbiology, 151: 1127-1138.

  • V1-131V

    H. Ma, B. Kumar, U. Ditges, Gunzer F, J. Buer and  A.-P. Zeng (2004) An extended transcriptional regulatory network of Escherichia coli and analysis of its hierarchical structure and network motifs. Nucleic Acids Res. 32: 6643-6649

  • V1-132V

    H. Ma, J. Buer and A.-P. Zeng (2004) Hierarchical structure and modules in the Escherichia coli transcriptional regulatory network revealed by a new top-down approach. BMC Bioinformatcs, 5:199.

  • V1-133V

    J. Sun, R. Daniel, I. Wagner-Döbler and A.-P. Zeng (2004) Is autoinducer-2 a universal signal for interspecies communication? A comparative and phylogenetic genomic analysis of the synthesis and signal transduction pathways. BMC Evolutionary Biology, 4:36.

  • V1-134V

    J Sun and A.-P. Zeng (2004) IDENTICS - Identification of coding sequences and in silico reconstruction of the metabolic network directly from unannotated low-coverage bacterial genome sequences. BMC Bioinformatics, 5:112.

  • V1-135V

    U Ditges, M Hartmann, S Coldewey, HW Ma, AP Zeng, F Gunzer (2004) Acid resistance in enterohemorrhagic E-coli: Is there a link to the serotype? International Journal of Medical Microbiology 294:161-161 Suppl. 39.

  • V1-136V

    H. Ma, X.-M. Zhao, Y-J. Yuan and A.-P. Zeng (2004) Decomposition of metabolic network into functional modules based on the global connectivity structure of reaction graph. Bioinformatics. 20: 1870-1876

  • V1-137V

    H. Ma and A.-P. Zeng (2004) Phylogenetic comparasion of metabolic capacities of organisms at genome level. Molecular Phylogenetics and Evolution. 31, 204-213.

  • V1-138V

    F. He, H. Ma, X.-M. Zhao, Y.-J. Yuan, A.-P. Zeng (2004) Progress and perspective in the application of bioinformatics for the analysis of metabolic networks. J. Chem. Ind.  Eng. (Chinese). 55:1593-1601.

  • V1-139V

    A.-P. Zeng and E.-J. Kim (2004) Iron availability, oxygen limitation, Pseudomonas aeruginosa and cystic fibrosis. Microbiology. 150: 516-518.

  • V1-140V

    Z.-L. Xiu, B.-H. Song, Z.-T. Wang, L.-H. Sun, E.-M. Feng and A.-P. Zeng (2004) Optimization of dissimilation of glycerol to 1,3-propanediol by Klebsiella pneumoniae in one- and two-stage anaerobic cultures. Biochem. Eng. J., 19: 189¿197

  • V1-141V

    H. Ma and A.-P. Zeng (2003) The connectivity structure, giant strong component and centrality of metabolic networks. Bioinformatics. 19:  1423-1430

  • V1-142V

    H. Ma and A.-P. Zeng (2003) Reconstruction of metabolic networks from genome data and analysis of their global structure for various organisms. Bioinformatics. 19: 270-277.

  • V1-143V

    W. Wang, J. Sun, M. Nimtz, W.-D. Deckwer and A.-P. Zeng (2003) Protein identification from 2-dimentional gel electrophoresis analysis of Klebsiella pneumoniae by combined use of mass spectrometry data and unannotated genome sequences. Proteome Science. 1, 6.

  • V1-144V

    J. Sun, J. van den Heuvel, P. Soucaille and A.-P. Zeng (2003) Comparative genomic analysis of dha regulon and related genes for anaerobic glycerol metabolism in microorganisms. Biotechnol. Prog. 19: 263-272.

  • V1-145V

    W. Wang, J. Sun, M. Hartlep, W.-D. Deckwer and A.-P. Zeng. (2003) Combined use of proteomic analysis and enzyme activity assay for metabolic pathway analysis of glycerol fermentation by Klebsiella pneumoniae. Biotechnol. Bioeng. 83: 525-536.

  • V1-146V

    J.-J. Qiao, Y.-J. Yuan, H. Zhao1, J.-C. Wu and A.-P. Zeng (2003) Apoptotic cell death in suspension cultures of Taxus cuspidata co-treated with salicylic acid and hydrogen peroxide. Biotechnology Letters 25: 387¿390.

  • V1-147V

    Qiao JJ, Zhao H, Ge ZQ, Yuan YJ, Zeng AP (2003). 2-dimensional gel electrophoresis on protein patterns from suspension cultures of Taxus cuspidate induced by SA.
    Chinese Journal of Biotechnology.  19(1): 92-96

  • V1-148V

    E.-J. Kim, W. Sabra, A.-P. Zeng (2003) Iron deficiency leads to inhibition of oxygen transfer and enhanced formation of virulence factors in Pseudomonas aeruginosa cultures. Microbiology 149, 2627-2634.

  • V1-149V

    J.-X. Bi, P. Buhr, A.-P. Zeng and M. Wirth (2003) The Human c-fos Promoter Mediates High-level, Inducible Expression in Various Mammalian Cell Lines. Biotechnol. Bioeng. 81: 845-854.

  • V1-150V

    W. Sabra, H. Lünsdorf and A.-P. Zeng (2003) Alterations in the formation of lipopolysaccharide and membrane vesicle on the surface of Pseudomonas aeruginosa PAO1 under oxidative stress conditions. Microbiology. 149, 2789-2795.

  • V1-151V

    J. Bi, M. Wirth, C. Beer, E.-J. Kim and A.-P. Zeng (2002) Dynamic characterization of recombinant Chinese Hamster Ovary cells containing an inducible c-fos promoter GFP expression system as a biomarker. J. Biotechnol. 93: 231-242.

  • V1-152V

    A.-P. Zeng and H. Biebl (2002) Bulk-Chemicals from Biotechnology: the case of microbial production of 1,3-propanediol and the new trends. In K. Schügerl and A.-P. Zeng (Eds.) Tools and Applications of Biochemical Engineering Science. Adv. Biochem. Eng. Biotechnol. Vol. 74: 237-257.

  • V1-153V

    Z.-Y. Ma; Y.-J. Yuan; J.-C. Wu and A.-P. Zeng (2002) Apoptotic cell death in suspension cultures of Taxus chinensis var. mairei. Biotechnol. Letters 24(7): 573-577.

  • V1-154V

    Y.-J. Yuan, Z.-Y. Ma, J.-C. Wu and A.-P. Zeng (2002) Taxol-induced apoptotic cell death in suspension cultures of Taxus cuspidate. Biotechnol. Letters 24(8): 615-618.

  • V1-155V

    Yuan, Y.-J., Li, C., Hu, Z.-D., J-C. Wu, Zeng, A.-P. (2002) Fungal Elicitor-Induced Cell Apoptosis in Suspension Cultures of Taxus chinensis var. mairei for Taxol Production. Process Biochem. 38: 193-198.

  • V1-156V

    Xiu, Z.-L., Song , B.-H., Sun, L.-H. and Zeng, A.-P. (2002) Theoretical analysis of effects of metabolic overflow and time delay on the performance and dynamic behavior of a two-stage fermentation process. Biochemical Eng. J. 11, 101-109.

  • V1-157V

    Xiu, Z.-L., Chang, Z.-Y. and Zeng, A.-P. (2002) Nonlinear dynamics of regulation of bacterial trp operon: model analysis of integrated effects of repression, feedback inhibition and attenuation. Biotechnol. Progress. 18. 686-693

  • V1-158V

     A.-P. Zeng, J. Modak and W.-D. Deckwer (2002) Nonlinear dynamics of eucaryotic pyruvate dehydrogenase multi-enzyme complex: Decarboxylation rate, oscillations and multiplicity. Biotechnol. Prog. 18: 1265-1276

  • V1-159V

    J. Modak, W.-D. Deckwer and A.-P. Zeng (2002) Metabolic control analysis of eucaryotic pyruvate dehydrogenase multi-enzyme complex. Biotechnol. Prog. 18: 1157-1169.

  • V1-160V

    W. Sabra, E.J. Kim and A.-P. Zeng (2002) Physiological responses of Pseudomonas aeruginosa PAO1 to oxidative stress in controlled microaerobic and aerobic culture.  Microbiology. 148: 3195-3202.

  • V1-161V

    M. Hartlep, W. Hussmann, N. Prayitno, I. Meynial-Salles and A.-P. Zeng (2002) Study of two-stage processes for the microbial production of 1,3-propanediol from glucose. App. Microbial. Biotechnol. 60:60-66.

  • V1-162V

    Sabra W., Ma H., Wang W., Kim E.-J., Zeng A.-P. (2002) Rekonstruktion und Simulation des metabolischen Netzwerks von Pseudomonas aeruginosa für die Bildung von Virulenzfaktoren. Chem.-Ing. Tech. 74: 688.

  • V1-163V

    Wael Sabra, A.-P. Zeng and W.-D. Deckwer (2001) Microbial alginates: physiology, quality and process aspects. Appl. Microbial. Biotechnol. 56: 315-325.

  • V1-164V

    Yuan, Y.-J., Ma Z.Y., Ge Z.Q, Miao Z.Q., Zeng, A.-P. (2001) Apoptosis and taxol production in suspension cultures of Taxus spp. cells. J. Chem. Ind. Eng.  51, 43-46.

  • V1-165V

    Wael Sabra, A.-P. Zeng, H. Lünsdorf and W.-D. Deckwer (2000) Effect of Oxygen on the Formation and Structure of Azotobacter vinelandii Alginate and its Role in Protecting Nitrogenase. Appl. Environ. Microbiol. 66 (9): 4037-4044.

  • V1-166V

    Z.-L. Xiu, A.-P. Zeng,  L.J. An and W.-D. Deckwer (2000) Transient responses and mathematical modeling of Klebsiella pneumoniae to dilution rate and glycerol concentration step changes in continuous culture. J. Chemical Engineering of Chinese Universities. Vol. 14, No.1: 53-58.

  • V1-167V

    A.-P. Zeng and M. Linz (2000) Programmed cell death (apoptosis) in animal cell culture: physiological and kinetic aspects. In: J.J. Zhong (ed.): Advances in Applied Biotechnology. ECUST Press, Shanghai. pp. 64-77.

  • 2-09.008V

    Becker, Peter: Der biologische Abbau von Fetten durch aerobe, thermophile Bakterien: Untersuchungen zur Kinetik und zum Einsatz in der Abwassertechnik, 1999

  • 2-09.018V

    Fuchs, C.: Einsatz einer Dialysemembran bei der Fermentation von E. coli. 2002

Dissertationen

  • 2-09.002D

    Lüdemann, Ines: "Nutrient-Split"-Fütterungsstrategie für suspendierte und immobilisierte nicht-adhärente tierische Zellen, 1997

  • 2-09.009D

    Fassnacht, Dieter: Fixed-bed reactors for the cultivation of animal cells, 2000

  • 2-09.010D

    Schwabe, Jan-Oliver: Feeding Strategies for Fed-Batch Cultures of Animal Cells, 2000

  • 2-09.024D

    Frahm, B.: Adaptive, modellgestützte Prozessführung von Suspensionskulturen tierischer Zellen. 2003

  • 2-09.025D

    Nagel-Heyer, St.: Ingenieurtechnische Aspekte bei der Herstellung von dreidimensionalen Knorpel-Träger-Konstrukten. 2004

  • 2-09.001D

    Reinhold, Georg: Maßstabsvergrößerung eines Biogas-Turmreaktors zur anaeroben Abwasserreinigung, 1996

  • 2-09.002D

    Lüdemann, Ines: "Nutrient-Split"-Fütterungsstrategie für suspendierte und immobilisierte nicht-adhärente tierische Zellen, 1997

  • 2-09.003D

    Krahe, Martin: Kultivierung von Mikroorganismen zu hohen Zelldichten, 1997.

  • 2-09.004D

    Müller, Birgit: Untersuchungen zum Abbau polyzyklischer aromatischer Kohlenwasserstoffe (PAK) durch thermophile Mikroorganismen, 1998.

  • 2-09.005D

    Polomski, Andreas: Einfluß des Stofftransports auf die Methanbildung in Biogas-Reaktoren, 1998

  • 2-09.006D

    Feitkenhauer, Heiko: Biodegradation of aliphatic and aromatic hydrocarbons at high temperatures, 1998

  • 2-09.007D

    Pietsch, Torsten: Rückhalt suspendierter Biomasse und örtliche Verteilung gelöster Gase in einem Biogas-Turmreaktor, 2000

  • 2-09.019D

    Köster, D.: Proteinexpression in dichten Kulturen von E. coli. 2002

  • 2-09.020D

    Biller, K.: Fermentation hyperthermophiler Mikroorganismen. 2002

  • 2-09.022D

    Hansen, G.: Der Venturi-Bioreaktor: Ein System mit hoher Stoffaustauschleistung zwischen Gas- und Flüssigphase. 2001

  • 2-09.023D

    Reimann, I.: Thermophile Behandlungsverfahren zur biologischen Reinigung fetthaltiger Abwässer. 2003

  • 2-09.026D

    Brodersen, J.: Biotechnologische Verfahren zum Abbau von Federn

  • 2-09.027D

    Göpel, Ph.: Pektinolytische Enzyme aus thermophilen Bakterien: Wirkungsweise auf Bastfasern, Charakterisierung und Produktion. 2006

Habilitationen

  • 2-09.001H

    Pörtner, R.: Reaktionstechnik der Kultur tierischer Zellen. 1997