Technical strategies to improve tissue engineering of cartilage carrier constructs
 The loss and damage of tissues cause serious health problems. As the existing therapies are not sufficient new therapy concepts for practical medical applications are required. To this end, tissue engineered substitutes generated in vitro could open up new strategies for the restoration of damaged tissues. Formation of 3D tissue substitutes in vitro requires not only a biological model (e.g., an adequate source for proliferable cells with appropriate biological functions, a protocol for proliferating cells while maintaining the tissue-specific phenotype), but also the further development of new culture strategies including bioreactor concepts. Within this project, the formation of cartilage-carrier constructs is used to demonstrate that the quality of the tissue can be significantly improved by using optimized culture conditions ( e.g. oxygen concentration, growth factor combination) as well as bioreactor techniques. It is assumed that mimicking in vivo loadings of the knee joint (fluid-dynamic, hydrostatic or mechanical load) by special bioreactors might increase matrix synthesis and organization of in vitro engineered cartilage-carrier constructs.The research activities are done in close cooperation with the Institute of Biomechanics (Prof. Morlock, Prof. Schilling, TUHH) and the University Hospital Eppendorf (PD Dr. Adamietz, Prof. Amiling, Prof. Meenen) and is integrated in the research group ¿Tissue Engineering¿ and the research center ¿Regeneration, Implants, Medical Technology¿ at TUHH. Furthermore the group of Prof. Becker, Uni Stuttgart-Hohenheim is partner within the DFG-project. Publikationen - 2-09.095V
Nagel-Heyer, St. and R. Pörtner: Bioreactor cultivation for three-dimensional cartilage implants. G.I.T. Laboratory Journal 2: 66-67 (2002) - 2-09.025D
Nagel-Heyer, St.: Ingenieurtechnische Aspekte bei der Herstellung von dreidimensionalen Knorpel-Träger-Konstrukten. 2004 - 2-09.105V
Nagel-Heyer, S.; R. Janßen; C. Goepfert; P. Adamietz; N.M. Meenen and R. Pörtner: Carti-lage cultivation on a calcium phosphate ceramic in a bioreactor system. Key Engineering Materials 264-268: 2119-2122 (2004) - 2-09.106V
Janssen, R.; S. Nagel-Heyer; Ch. Goepfert; R. Pörtner; D. Toykan; O. Krummhauer; M.M. Morlock; P. Adamietz; N.M. Meenen; W.M. Kriven; D.-K. Kim; A. Tampieri and G. Celotti: Cal-cium phosphate ceramics as substrate for cartilage cultivation. Ceramic Engineering and Sci-ence Proceedings 25, 4: 523-528 (2004) - 2-09.108V
Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters, 27(3): 187-192 (2005) - 2-09.110V
Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters 27(3): 187-192 (2005) - 2-09.112V
Nagel-Heyer, S.; Ch. Goepfert; P. Adamietz; N.M. Meenen; J.-P. Petersen and R. Pörtner: Flow-chamber bioreactor culture for generation of three-dimensional cartilage-carrier-constructs. Bioprocess and Biosystems Engineering, 27: 273-280 (2005) - 2-09.113V
Meenen, N.M., P. Ueblacker, R. Pörtner, C. Göpfert, S. Nagel-Heyer, J. P. Petersen und P. Adamietz: ; „ Knorpel aus dem Labor-eine Sackgasse?“,. Arthroskopie 18: 245-252 (2005) - 2-09.114V
Pörtner, R.; St. Nagel-Heyer; Ch. Goepfert; P. Adamietz and N. M. Meenen: Bioreactor design for tissue engineering. Journal of Bioengineering and Bioscience, 100(3): 235-245 (2005) - V-1.003V
Nagel-Heyer, St.; Ch. Goepfert; P. Adamietz; N. M. Meenen and R. Pörtner: Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension. Journal of Biotechnology, 121 (4), 486-497 (2006) - V-1.005V
Heyland, J.; K. Wiegandt; Ch. Goepfert; St. Nagel-Heyer; E. Ilinich; U. Schumacher and R. Pörtner: Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure. Biotechnology Letters, 28: 1641-1648 (2006) - V-1.007V
Petersen, J.-P.; P. Uebelacker; Ch. Goepfert; P. Adamietz; A. Stork; J.M. Rueger; R. Pörtner; M. Amling and N.M. Meenen: Long term results after implantation of tissue engineered cartilage for the treatment of osteochondral lesions in a minipig model. Journal of Materials Science: Materials in Medicin, DOI: 10.1007/s10856-007-3291-3
Stichwörter - Bioreactor
- Cartilage
- Tissue Engineering
- ceramic carrier
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